In vitro free radical scavenging and thrombolytic activities of Bangladeshi aquatic plant Aponogeton undulatus Roxb

Abstract

Investigation with the crude methanolic extract of Aponogeton undulatus was carried out to evaluate its possible antioxidant and thrombolysis activity. In DPPH free radical scavenging assay, the extract exhibited potent antioxidant activity with a IC50 values of 2.43±1.06 μg/ml while in ascorbic acid, the value become 50 2.14±0.11 μg/ml. In thrombolytic activity using in vitro clot lysis assay method, the crude methanolic extract was found to have significant (p<0.001) thrombolytic activity at a dose of 10 mg/ml with a miximum effect of 20.23±1.56% while the standard streptokinase showed 46.13±3.87%. The extract was also investigated for its antibacterial and toxic potentiality using agar diffusion and Brine Shrimp lethality bioassay, respectively. The highest antibacterial effect was shown against Bacillus cereus (zone of inhibition 12±0.65 mm) followed by Escherichia coli (zone of inhibition 10±0.71 mm). In this bioassay the extract showed significant toxicity to Brine Shrimp nauplii with the LC50 value of 2.24±0.98 μg/ml. The study clearly indicated that the extract 50 possesses good antioxidant and thromolytic activity along with broad spectrum antibacterial and toxic potentiality.