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In vitro free radical scavenging and thrombolytic activities of Bangladeshi aquatic plant Aponogeton undulatus Roxb
(© IDOSI Publications, 2011, 2011) Chowdhury, Nargis S; Alam, Md. Badrul; Haque, A S M Tanbirul; Zahan, Ronok; Ehsanul, M; Mazumder, Haque; Haque, M Ekramul; Department of Pharmacy
Investigation with the crude methanolic extract of Aponogeton undulatus was carried out to evaluate its possible antioxidant and thrombolysis activity. In DPPH free radical scavenging assay, the extract exhibited potent antioxidant activity with a IC50 values of 2.43±1.06 μg/ml while in ascorbic acid, the value become 50 2.14±0.11 μg/ml. In thrombolytic activity using in vitro clot lysis assay method, the crude methanolic extract was found to have significant (p<0.001) thrombolytic activity at a dose of 10 mg/ml with a miximum effect of 20.23±1.56% while the standard streptokinase showed 46.13±3.87%. The extract was also investigated for its antibacterial and toxic potentiality using agar diffusion and Brine Shrimp lethality bioassay, respectively. The highest antibacterial effect was shown against Bacillus cereus (zone of inhibition 12±0.65 mm) followed by Escherichia coli (zone of inhibition 10±0.71 mm). In this bioassay the extract showed significant toxicity to Brine Shrimp nauplii with the LC50 value of 2.24±0.98 μg/ml. The study clearly indicated that the extract 50 possesses good antioxidant and thromolytic activity along with broad spectrum antibacterial and toxic potentiality.
Antioxidant, analgesic and toxic potentiality of stephania japonica (Thunb.) miers. leaf
(© 2011 Asian Network for Scientific Information., 2011) Rahman, Md Habibur; Alam, Md. Badrul; Chowdhury, N.S.; Kumar Jha, Mithilesh; M, Hasan; Khan, Md. Maruf; Rahman, Md. Saifur; Haque, M. Ekramul; Department of Pharmacy
In the present study crude methanolic extract of Stephania japonica leaf was investigated for possible antioxidant, analgesic and toxic activity. The extract showed antioxidant activity in DPPH radical scavenging activity, nitric oxide scavenging activity and reducing power assays. In both DPPH radical and NO scavenging assay, the extract exhibited moderate antioxidant activity and the IC50 values in DPPH radical scavenging and NO scavenging assays were found to be 105.55±1.06 and 129.12±0.15 ug mL-1, respectively while the IC50 values of ascorbic acid were 12.30±0.11 and 18.64±0.22 ug mL-1, respectively. Reducing power activity of the extract increased in a dose dependent manner. Analgesic activity of the crude extract was evaluated using acetic acid-induced writhing model of pain in mice. The crude extract at 200 and 400 mg kg-1 b. wt. doses displayed significant (p<0.001) reduction in acetic acid induced writhing in mice with a maximum effect of 75.89% reduction at 400 mg kg-1 b.wt. which is comparable to the standard, diclofenac sodium (86.52%). The extract was also investigated for toxic potentiality using Brine Shrimp lethality bioassay. In this bioassay the extract showed significant toxicity to Brine Shrimp nauplii with the LC50 value of 25.19±0.98 ug mL-1. The study clearly indicates that the extract possesses good analgesic and cytotoxic activity along with moderate antioxidant potential.
Antioxidant, analgesic and anti-inflammatory activities of the herb Eclipta Prostrate
(© 2011 Academic Journals Inc., 2011) Hossain, M.S.; Alam, M.B.; Chowdhury, N.S.; Asadujjaman, M.; Zahan, R.; Islam, M.M.; Mazumder, M.E.H.; Haque, M.E.; Islam, A.; Department of Pharmacy
The present study was designed to investigate the antioxidant, analgesic and anti-inflammatory potential of the methanolic extract along with its organic soluble fractions of the herb Eclipta prostrata. In addition, total phenolic and flavonoid content and total antioxidant capacity were also determined. Antioxidant potential of the extract/fractions was evaluated by DPPH (1,1diphenyl-2-picrylhydrazyl), NO (nitric oxide) and ONOO- (peroxynitrite) scavenging assay method. Ethyl acetate fractions (EtOAc) showed highest scavenging activity in all the methods with IC50 value of 12.98±0.08, 45.98±0.07 and 14.45±0.18 μg mL-1 for DPPH, NO and ONOO- assay method, respectively. In reducing power assay, EtOAc fraction also showed significant (p<0.001) activity. Further, the extract/fractions were studied for their analgesic (hot plate, tail immersion and acetic acid induced writhing test) and anti-inflammatory (carrageenan induced paw edema in rats) activities at a dose level of 200 and 400 mg kg-1 body weight. Among all the extract/fractions, EtOAc fraction showed a dose dependent and significant (p<0.005, p<0.05) analgesic activity in all the tested method. EtOAc fractions also reduced the paw edema considerably (86.80% inhibition after 3 h, p<0.005, p<0.05) in dose dependent manner compared to carrageenan induced rat. Altogether, these results suggest that the MeOH extract and its organic soluble fractions could be used as a potential antioxidant, analgesic and anti-inflammatory agent
Antioxidant, anti-inflannnatory and anti-pyretic activities of trichosanthes dioica roxb. fruits
(© 2011 Academic Journals Inc., 2011) Alam, M. Badrul; Hossain, M. Sarowar; Chowdhury, N. Sultana; Asadujjaman, M.; Zahan, Ronok; Islam, M. Monirul; Mazumder, M. Ehsanul Haque; Haque, M. Ekramul; Islam, Anwarul; Department of Pharmacy
The present study was designed to investigate the antioxidant, anti-inflammatory and antipyretic potential of the methanolic extract along with its organic soluble fractions of the fruits of Trichosanthes dioica Roxb. Antioxidant potential of the extract/fractions was evaluated by DPPH (1,ldiphenyl-2-picrylhydrazyl) and NO (nitric oxide) scavenging assay method. Ethyl acetate fractions (EtOAc) showed highest scavenging activity in all the methods with IC50 value of 12.32±0.16 and 5.38±0.07 pg mL -1 for DPPH and NO assay method, respectively. In reducing power assay, EtOAc fraction also showed significant (p<O.OOl) activity. Further, the extract/fractions were studied for their anti-inflammatory (carrageenan induced paw edema in rats) and antipyretic (Brewer's yeast induced pyrexia) activities at a dose level of 100, 200 and 400 mg kg-1 body weight for MeOH extract. Methanolic extract showed a dose dependent and significant (p<0.005, p<0.05) anti -inflammatory and antipyretic effect. Dichloromethane fraction (CH2CI2) and Ethyl acetate fractions exhibited similar activity using a dose of 200 mg kg-1 b.wt. in these models. The pharmacological activities of the (CH2CI2) fraction were lesser than the MeOH extract and other fractions. In addition, total phenolic and flavonoid content and total antioxidant capacity were also determined. Altogether, these results suggest that the MeOH extract and its organic soluble fractions EtOAc could be used as a potential antioxidant, anti -inflammatory and antipyretic agent.
Anticancer activity of alangium salvifolium flower in ehrlich ascites carcinoma bearing mice
(© 2011 Academic Journals Inc., 2011-03) Zahan, Ronok; Alam, Md. Badrul; Islam, Saiful; S. Chowdhury, Nargis; B. Hosain, Salman; Mosaddik, Ashik; Jesmin, Mele; Haq, M. Ekramul; Department of Pharmacy
The research study was conducted to determine the antitumor effect of the flower of Alangium salvifolium (crude extract and diethylether fractions) against Ehrlich Ascites Carcinoma (EAC) in mice at the doses of 10 mg kg-1 body weight intraperitoneally. Extract/fractions was administered for nine consecutive days. Twenty-four hours of last dose and 18 h of fasting, the mice were sacrificed and antitumor effect was assessed by evaluating tumor volume, viable and nonviable tumor cell count, tumor weight and hematological parameters of EAC bearing host. Significant (p<0.001) increases of survival times 30±0.96 and 25±0.40 days for crude extract and diethylether fraction of the A. salvifolium (10 mg kg-1) treated tumor bearing mice, respectively were confirmed with respect to the control group (20±0.13 days). The extract/fraction also decreased the body weight of the EAC tumor bearing mice. Hematological studies reveal that the heamoglobin (Hb) content was decreased in EAC treated mice whereas restoration to near normal levels was observed in extract treated animals. There was a significant (p<0.001) decrease in RBC count and increase in WBC counts in extract/fraction treated animals when compared to EAC treated animals. From the result it was showed that the extract has significant anticancer activity and that is comparable to that of Bleomycin.
Anti-alzheimer and antioxidant activity of celastrus paniculatus seed
(© 2011 Iranian Journal of Pharmaceutical Sciences, 2011) Alam, Badrul; Haque, Ekramul; Department of Pharmacy
The crude methanolic extract of the seeds ofCelastrus paniculatus along with its organic soluble fractions were tested for their possible antioxidant and antialzheirmer (AD) activity. The extracts showed prominent DPPH free radical scavenging activity, inhibiting activity of authentic peroxynitrite (ONOO-) and inhibition of total reactive oxygen species (ROS) generation. In DPPH radical scavenging assay, the EtOAc fraction showed the highest activity with a IC50 value of 25.92±1.02 mg/ml whereas aqueous fractions had no activity at all within the tested concentration. Scavenging of the authentic ONOO- system, all extract/fractions showed good activity and among them, EtOAc fraction had the highest activity with a IC50 value of 15.79±0.18 mg/ml. EtOAc fraction also showed significant (p<0.001) inhibitory activity against the total ROS generation which was almost similar with that of the positive control Trolox (IC50 16.79±0.19mg/ml). All extract/fractions exhibited statistically significant (p<0.001) cholinesterases (ChEs) inhibitory effects with IC50 values ranging between 134.7-227.5 mg/ml for AChE and 209.6-562.1 mg/ml for BChE.

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