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    •   BracU IR
    • School of Data and Sciences (SDS)
    • Department of Mathematics and Natural Sciences (MNS)
    • Bachelor of Science in Microbiology
    • Thesis (Bachelor of Science in Microbiology)
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    Carbapenem resistant Escherichia coli and Klebsiella pneumoniae in community wastewaters of Dhaka, Bangladesh

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    18326031_MNS.pdf (334.7Kb)
    Date
    2021-12
    Publisher
    Brac University
    Author
    Shafi, Sazid Al
    Metadata
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    URI
    http://hdl.handle.net/10361/16359
    Abstract
    Background: Carbapenems are beta-lactams that include some of the most widely used antibiotics worldwide, with inhibition of its treatment posing an alarming risk to healthcare. Resistance to this drug is determined by the production of beta-lactamases which are encoded by genes such as NDM-1, IMP, VIM, OXA-48 etc. The detection of such carbapenem resistant bacterial species has been observed in both hospital and community wastewaters of Dhaka, Bangladesh. As microorganisms from sewage can find its way to drinking water, the spread of these microorganisms into community wastewater lines creates the possibility of infections that are challenging to treat. As such, this study was conducted on community wastewater samples to thoroughly monitor and determine the distribution of carbapenem-resistant bacteria in different community regions at differing distances. Materials and Methods: Wastewater samples were collected from 8 sites in Dhaka and transferred to the laboratory. Bacterial cultures were grown on selective agar media for isolation of Escherichia coli and Klebsiella pneumoniae colonies. Antibiotic resistance profiles of colonies were determined with Kirby-Bauer disc diffusion testing with PCR identification of beta-lactamase genes. Results: From this study it can be perceived that, 48.84% K. pneumoniae which were either imipenem or meropenem resistant or both of antibiotic resistant, most of them carried the blaNDM gene which is the most common gene responsible for coding the carbapenemase enzyme known as metallo beta-lactamase that makes bacteria resistant to a broad range of beta-lactam antibiotics. But, even though the prevalence of carbapenem resistant E. coli is higher (51.16%), blaNDM gene carriers were less in comparison to K. pneumoniae.
    Keywords
    Carbapenem; Antibiotic; Dhaka; blaNDM; E. coli; K. pneumoniae; Community wastewater
     
    LC Subject Headings
    Escherichia coli infections.; Sewage -- Purification
     
    Description
    This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Microbiology, 2021.
     
    Catalogued from PDF version of thesis.
     
    Includes bibliographical references (pages 12-14).
    Department
    Department of Mathematics and Natural Sciences, Brac University
    Collections
    • Thesis (Bachelor of Science in Microbiology)

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