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dc.contributor.advisorHaque, Fahim Kabir Monjurul
dc.contributor.authorShafi, Sazid Al
dc.date.accessioned2022-02-27T10:04:54Z
dc.date.available2022-02-27T10:04:54Z
dc.date.copyright2021
dc.date.issued2021-12
dc.identifier.otherID 18326031
dc.identifier.urihttp://hdl.handle.net/10361/16359
dc.descriptionThis thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Microbiology, 2021.en_US
dc.descriptionCatalogued from PDF version of thesis.
dc.descriptionIncludes bibliographical references (pages 12-14).
dc.description.abstractBackground: Carbapenems are beta-lactams that include some of the most widely used antibiotics worldwide, with inhibition of its treatment posing an alarming risk to healthcare. Resistance to this drug is determined by the production of beta-lactamases which are encoded by genes such as NDM-1, IMP, VIM, OXA-48 etc. The detection of such carbapenem resistant bacterial species has been observed in both hospital and community wastewaters of Dhaka, Bangladesh. As microorganisms from sewage can find its way to drinking water, the spread of these microorganisms into community wastewater lines creates the possibility of infections that are challenging to treat. As such, this study was conducted on community wastewater samples to thoroughly monitor and determine the distribution of carbapenem-resistant bacteria in different community regions at differing distances. Materials and Methods: Wastewater samples were collected from 8 sites in Dhaka and transferred to the laboratory. Bacterial cultures were grown on selective agar media for isolation of Escherichia coli and Klebsiella pneumoniae colonies. Antibiotic resistance profiles of colonies were determined with Kirby-Bauer disc diffusion testing with PCR identification of beta-lactamase genes. Results: From this study it can be perceived that, 48.84% K. pneumoniae which were either imipenem or meropenem resistant or both of antibiotic resistant, most of them carried the blaNDM gene which is the most common gene responsible for coding the carbapenemase enzyme known as metallo beta-lactamase that makes bacteria resistant to a broad range of beta-lactam antibiotics. But, even though the prevalence of carbapenem resistant E. coli is higher (51.16%), blaNDM gene carriers were less in comparison to K. pneumoniae.en_US
dc.description.statementofresponsibilitySazid Al Shafi
dc.format.extent14 pages
dc.language.isoenen_US
dc.publisherBrac Universityen_US
dc.rightsBrac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission.
dc.subjectCarbapenemen_US
dc.subjectAntibioticen_US
dc.subjectDhakaen_US
dc.subjectblaNDMen_US
dc.subjectE. colien_US
dc.subjectK. pneumoniaeen_US
dc.subjectCommunity wastewateren_US
dc.subject.lcshEscherichia coli infections.
dc.subject.lcshSewage -- Purification
dc.titleCarbapenem resistant Escherichia coli and Klebsiella pneumoniae in community wastewaters of Dhaka, Bangladeshen_US
dc.typeThesisen_US
dc.contributor.departmentDepartment of Mathematics and Natural Sciences, Brac University
dc.description.degreeB. Microbiology


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