dc.contributor.advisor | Naser, Iftekhar Bin | |
dc.contributor.author | Mahdee, Munif | |
dc.contributor.author | Abdullah, Syed Ridwan | |
dc.date.accessioned | 2024-06-05T04:17:41Z | |
dc.date.available | 2024-06-05T04:17:41Z | |
dc.date.copyright | 2023 | |
dc.date.issued | 2023-09 | |
dc.identifier.other | ID 19236007 | |
dc.identifier.other | ID 19236008 | |
dc.identifier.uri | http://hdl.handle.net/10361/23134 | |
dc.description | This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2023. | en_US |
dc.description | Catalogued from the PDF version of thesis. | |
dc.description | Includes bibliographical references (pages 43-44). | |
dc.description.abstract | Recently acquired bacteriophages from water samples in Dhaka city which degraded the
bacterial biofilms were characterized in this investigation. Bacteriophages unique to E. coli strain
9A(ⲫ9A) and Vibrio cholerae strain 13B(ⲫ13B) were identified. Throughout the process, strict
laboratory protocols were followed, and the characterization was based on three variables:
temperature, pH, and salinity. In the first step of the procedure, single colonies of host bacteria
were obtained by streak plating, which was followed by enrichment of the corresponding
bacteriophages. The Phage counts were then determined using the Double Layer Assay (DLA).
To begin DLA, single colonies in LB broth were first taken and cultured in a shaker. The
bacteriophages underwent a series of dilutions before the phages and the bacteria were added in
the molten soft agar. After being centrifuged, the soft agar was quickly placed onto a Luria
Bertani Agar plate. After evenly distributing the materials, the plate was swirled and until the
agar solidified and was then placed in an incubator for 16 hours. The following day, the plaques
were counted, and the number of Plaque Forming Units per Milliliter (PFU/ml) were determined.
An average phage count was discovered after the experiment was conducted three times for each
component. Both ⲫ9A and ⲫ13B displayed unusual results in temperature and pH readings in
addition to salinity. | en_US |
dc.description.statementofresponsibility | Munif Mahdee | |
dc.description.statementofresponsibility | Syed Ridwan Abdullah | |
dc.format.extent | 44 pages | |
dc.language.iso | en | en_US |
dc.publisher | Brac University | en_US |
dc.rights | Brac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. | |
dc.subject | Biofilm | en_US |
dc.subject | Lake water | en_US |
dc.subject | Dhaka city | en_US |
dc.subject.lcsh | Biotechnology. | |
dc.title | Characterization of newly identified bacterial biofilm degrading bacteriophage obtained from Dhaka city lake water samples | en_US |
dc.type | Thesis | en_US |
dc.contributor.department | Department of Mathematics and Natural Sciences, Brac University | |
dc.description.degree | B. Biotechnology | |