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dc.contributor.advisorAhmed, Akash
dc.contributor.authorPurkaystha, Anirban
dc.contributor.authorMegha, Sumiya Al Sadia
dc.date.accessioned2023-05-15T05:08:22Z
dc.date.available2023-05-15T05:08:22Z
dc.date.copyright2022
dc.date.issued2022-11
dc.identifier.otherID 18126047
dc.identifier.otherID 18126071
dc.identifier.urihttp://hdl.handle.net/10361/18286
dc.descriptionThis thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2022.en_US
dc.descriptionCatalogued from PDF version of thesis.
dc.descriptionIncludes bibliographical references (pages 36-38).
dc.description.abstractTextile and dyeing industry’s effluents and wastes are considered one of the top water pollutants in Bangladesh which ultimately leads to public health hazard. This study intends to explore the capacity of local bacteria to degrade dye and the roles of pH concentration and temperature in facilitating Azo dye and Reactive Dye degradation. Samples from river sludge were taken from the Dhaleshwari and Turag rivers and serially diluted. Then bacterial isolation was randomly selected based on morphology and each individual colony of focus was inoculated in dye-mixed nutritional broth. Colonies were cultured for seven days at various temperatures and had different pH concentrations to study biodegradability. To determine the actual decolourizationn (%) amount, optical density values were measured for each sample in the meanwhile. All microorganisms demonstrated successful biodegradability for selected dyes. Biochemical tests were carried out for the identification of the bacterial strains. This study found that Bacillus spp. degraded azo dye at a rate of 69.26% at pH 6 & 37 ̊c. Furthermore, Bacillus spp. Attained 52.70 % degradation at pH 6 and 45 °c. The results disclosed that Bacillus spp. were the most efficient in degrading reactive and azo dyes, followed by Bacillus subtilis and Staphylococcus hominis. This study can further instigate the scope of the biodegradability of dye to be ready for commercial usage for effluent treatment.en_US
dc.description.statementofresponsibilityAnirban Purkaystha
dc.description.statementofresponsibilitySumiya Al Sadia Megha
dc.format.extent38 pages
dc.language.isoenen_US
dc.publisherBrac Universityen_US
dc.rightsBrac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission.
dc.subjectDegradationen_US
dc.subjectDecolourizationen_US
dc.subjectBiodegradabilityen_US
dc.subjectBacillus spp.en_US
dc.subjectBacillus subtilisen_US
dc.subjectStaphylococcus hominisen_US
dc.titleIsolation, identification and efficacy assessment of bacteria degrading azo dye & reactive dye from river sludge sampleen_US
dc.typeThesisen_US
dc.contributor.departmentDepartment of Mathematics and Natural Sciences, Brac University
dc.description.degreeB. Microbiology


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