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    •   BracU IR
    • School of Data and Sciences (SDS)
    • Department of Mathematics and Natural Sciences (MNS)
    • Bachelor of Science in Microbiology
    • Thesis (Bachelor of Science in Microbiology)
    • View Item
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    Prevalence of Carbapenem-resistant Klebsiella pneumoniae in vegetable and water samples in Dhaka South

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    16226013, 16236012, 17126030, 17126032_MNS.pdf (3.319Mb)
    Date
    2022-03
    Publisher
    Brac University
    Author
    Raymond, Mugenyi
    Precious, Bisoboka Claire
    Haque, Ashrin
    Rahman, Mohammad Mushfiqur
    Metadata
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    URI
    http://hdl.handle.net/10361/17567
    Abstract
    Carbapenems are commonly considered last-resort antibiotics in the treatment of severe infections caused by multidrug-resistant Enterobacteriaceae. The emergence of carbapenem-resistant isolates over the last decade has been a major concern as it has contributed to narrowing the current treatment palette, leaving few or in some cases optimal treatment options. The present research was done to isolate and assess the prevalence of carbapenem-resistant Klebsiella pneumoniae in vegetable and water samples from hospital adjacent areas in Dhaka South. In this study, 55 vegetable and 50 water samples were randomly collected from different local markets near hospitals in Dhaka South. These samples were collected from September 2021 to November 2021. Out of 606 strains isolated throughout the study (from both enrichment and without enrichment cultures), 77 (12.71%) were Klebsiella pneumoniae. These isolates were subjected to an antibiotic susceptibility test using the following 12 antibiotics; Meropenem+EDTA (MR+ED), Imipenem (IMI10), Kanamycin(K30), Gentamicin (CN10), Ceftazidime (CAZ30), Erythromycin(E15), Nitrofurantoin (NIT300), Ampicillin (AMP10), Amoxyclav (AMC30), Nalidixic acid (NA30), Streptomycin(S10), and Tetracycline (TE30). All the 77 Klebsiella pneumoniae isolates were found to be resistant to Meropenem + EDTA (100%), followed by Nalidix Acid and Erythromycin to which 72.70% and 63.60% were resistant, respectively. This was then followed by Kanamycin (57.10%), Ampicillin (55.80%), Ceftazidime (51.90%), Nitrofurantoin (49.40%), Tetracycline (33.80%), Streptomycin (18.20%), Imipenem (15.60%), Gentamicin (10.40%) and Amoxyclav (7.80%). 4 (5.19%) of the K. pneumoniae isolates were resistant to all the 12 tested antibiotics. When the Multiple Antibiotic Resistance (MAR) index was calculated, 5(6.49%), 39(50.65%), 29(37.66%), and 4(5.19%) were identified under MAR indices <0.2, 0.2-<0.5, 0.5-<1 and 1 respectively. PCR was used to detect carbapenemase genes; blaNDM-1 and blaOXA-2 in 59 DNA-extracted K. pneumoniae isolates. 5(8.47%) of the isolates were positive for the blaNDM-1 gene, out of which, 3 and 2 came from samples collected from Anandabazar and Moulovibazar respectively. On the other hand, 9(15.25%) of the isolates were positive for the blaOXA-2 gene, out of which, 5, 3, and 1 came from samples collected from Anandabazar, Moulovibazar, and Moghbazar respectively. The findings of the current study demonstrated the potential of vegetables and water from markets located in hospital adjacent areas of Dhaka South, to be reservoirs of carbapenem-resistant - multidrug-resistant bacteria
    Keywords
    Carbapenem-Resistant Enterobacteriaceae; bla-NDM1; bla-OXA2; Klebsiella pneumoniae; Hospital Adjacent Area
     
    LC Subject Headings
    Bacterial diseases.; Pneumonia.
     
    Description
    This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2022.
     
    Catalogued from PDF version of thesis.
     
    Includes bibliographical references (pages 64-77).
    Department
    Department of Mathematics and Natural Sciences, Brac University
    Collections
    • Thesis (Bachelor of Science in Microbiology)

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