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    • Thesis (Bachelor of Science in Microbiology)
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    •   BracU IR
    • School of Data and Sciences (SDS)
    • Department of Mathematics and Natural Sciences (MNS)
    • Bachelor of Science in Microbiology
    • Thesis (Bachelor of Science in Microbiology)
    • View Item
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    Prevalence and diversity of antimicrobial resistant pathogens from medical and community wastewater in Dhaka City

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    18126058, 18126057, 18126015_MNS.pdf (1.191Mb)
    Date
    2022-05
    Publisher
    Brac University
    Author
    Bulbuli, Naima Hasan
    Awysharjaw, Rownak Showkat
    Lohani, Tabassum
    Metadata
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    URI
    http://hdl.handle.net/10361/17531
    Abstract
    Background: Uncontrolled and excessive exposure of antibiotics in the medical waste poses a selective pressure to acquire and disseminate highly antibiotic resistance genes to common pathogenic bacteria. This study is aimed to isolate common pathogenic bacteria and prevalence of multiple drug resistance genes in community and hospital wastewater. Materials and Methods: Ten different wastewater samples (5 hospitals and 5 community wastewater) were aseptically collected and bacteriological investigations were performed for quantification and identification of bacterial isolates. Antibiotic susceptibility was performed by the Kirby-Bauer disk diffusion method and interpreted using the CLSI guideline. Polymerase chain-reaction (PCR) will be performed for identification of ESBL and carbapenem resistance genes. Results: Out of 234 isolates collected (based on different morphology); Gram-negative bacteria were predominant (85.04%). Top pathogenic-bacteria isolates were E.coli-(15%); Klebsiella pneumonia (12.82%); Pseudomonas aeruginosa (8.11%); Salmonella sp. (7.26%); Shigella sp. (6.84%); Staphylococcus aureus (6%). Overall 76.92% isolates were resistant to ampicillin and 34.61%, 40%, 23.08% and 12.39% were resistant to third-generation cephalosporin, vancomycin, colistin and carbapenem respectively. From overall isolates, the MDR and XDR organisms were found to be 84.62% and 5.13% respectively. PCR data on ESBL and carbapenem resistance genes are ongoing. Conclusion: High prevalence of multiple-drug-resistance bacteria in both hospital and community wastewater is indicating the dissemination of these pathogens in community which may lead to great public health issues if the situation is not tackled in near future.
    Keywords
    Pathogens; Antibiotic; Dhaka; Hospital wastewater; Community wastewater; Multi Drug Resistance
     
    LC Subject Headings
    Medical wastes; Medical centers--Waste disposal
     
    Description
    This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2022.
     
    Catalogued from PDF version of thesis.
     
    Includes bibliographical references (pages 31-32).
    Department
    Department of Mathematics and Natural Sciences, Brac University
    Collections
    • Thesis (Bachelor of Science in Microbiology)

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