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dc.contributor.advisorSeraj, Dr. Zeba Islam
dc.contributor.advisorIslam, Dr. Aparna
dc.contributor.authorRazzaque, Samsad
dc.date.accessioned2012-03-14T05:38:43Z
dc.date.available2012-03-14T05:38:43Z
dc.date.copyright2011
dc.date.issued2011-10
dc.identifier.otherID 10176007
dc.identifier.urihttp://hdl.handle.net/10361/1665
dc.descriptionThis thesis report is submitted in partial fulfillment of the requirement for the degree of Masters of Science in Biotechnology, 2011
dc.descriptionCataloged from PDF version of thesis report.
dc.descriptionIncludes bibliographical references (page 84-87).
dc.description.abstractThe costal belt of Bangladesh is under the threat of increasing salinity and thus reducing plant productivity. Consequently, salinity has become a major concern for ensuring food security. Salt tolerance is known to be a complex trait both genetically as well as physiologically and conferring this tolerance by introducing a single gene is therefore difficult. However, over expression of the vacuolar antiporter gene, NHX1 has been reported to provide salinity tolerance to a good extent. This work was carried out to clone the coding sequence of the rice antiporter gene (applying Gateway Technology) in a binary vector (pH7WG2.0) from where this can be easily introduced into a plant genome with a highly efficient constitutive promoter (CaMV35S). In this study, NHX 1 gene was first cloned into pENTR and confirmed the insertion through PCR, Restriction Digestion and Sequencing technique. Then the gene of interest was recombined from pENTR to the Destination vector (pH7WG2.0) and recombination was confirmed by PCR and Restriction Digestion respectively. Finally, recombinant vector was then transformed into Agrobacterium (the transformation was also confirmed by PCR and Restriction Digestion) to perform Agrobacterium mediated transformation into tomato plant.en_US
dc.description.statementofresponsibilitySamsad Razzaque
dc.format.extent87 pages
dc.language.isoenen_US
dc.publisherBRAC Universityen_US
dc.rightsBRAC University thesis are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission.
dc.subjectBiotechnology
dc.titleCloning of an antiporter gene (NHX1) from PCR amplicons into a recombination competent vector containing a constitutive promoter (caMV35S)en_US
dc.typeThesisen_US
dc.contributor.departmentDepartment of Mathematical and Natural Science, BRAC University
dc.description.degreeM. Biotechnology


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