In Silico structural analysis of the complex PSKR receptor like Kinase, Pamp Phytosulfokine and SERk1 co-receptor
![Thumbnail](/xmlui/bitstream/handle/10361/15012/16136024_MNS.pdf.jpg?sequence=4&isAllowed=y)
View/ Open
Date
2021-02Publisher
Brac UniversityAuthor
Thakur, Hasnain NizamMetadata
Show full item recordAbstract
Plants often have to undergo pathogen invasions in nature. Nevertheless, as a result of
proficient immune systems owned by the host plants, plant diseases seldom occur. In the
process two separate recognition mechanisms identify pathogens, and one of them is known as
pattern-triggered immunity (PTI) which plays an essential role in plant defense system. PRRs
preliminarily conjoin to the pathogen-associated molecular patterns (PAMPs) in the case of a
microbial incursion and then go on to ever further assigned co-receptor proteins to stimulate
the defensive signal and optimize the immune response of the plant. While many plant PRRs
have been found, only few have been thoroughly described and their structural specifications
have been analyzed. In this analysis, the PSKR-Phytosulfokine-SERk1 (PDB ID: 4z64)
complex crystallographic structure was simulated for 30 ns at several phases, as there were five
different composites of the prime crystallographic composition. In order to obtain a synopsis
of the interaction and immune responsiveness of PSKR against Phytosulfokine with the aid of
co-receptor SERk1, 30ns of simulated trajectories were studied for each amalgamation.
In addition, it was found here that from Phytosulfokine, Thr31 and Gln32 made a notable con
tribution to the PSKR-Phytosulfokine complex development. It indicates that Phytosulfokine
interacts predominantly with a β-strand from the PSKRR island domain and the development
of an anti- β -sheet takes place in the process. Therefore, any alteration to the PAMP at these
positions can be expected to be dramatically pernicious to the plant, resulting in the PRR failing
its ability to detect the PAMP. As PSKR has been observed to play a key role in the plant
defense mechanism of Arabidopsis thaliana, its theorized binding method with Phytosulfokine
and SERk1 co-receptor can guide to draw a clearer picture of PTI's initial phases.