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    • Thesis (Bachelor of Science in Microbiology)
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    •   BracU IR
    • School of Data and Sciences (SDS)
    • Department of Mathematics and Natural Sciences (MNS)
    • Bachelor of Science in Microbiology
    • Thesis (Bachelor of Science in Microbiology)
    • View Item
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    Isolation of bacteriophages from surface waters specific for Shigella dysenteriae and their characterization as potential biocontrol agents

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    14126011_MNS.pdf (1.164Mb)
    Date
    2019-07
    Publisher
    Brac University
    Author
    Mubarak, Ayesha
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    URI
    http://hdl.handle.net/10361/13677
    Abstract
    Shigella is a low-infectious dose pathogen that can cause disease in healthy people. It causes a range of diseases from mild watery diarrhea to severe dysentery (shigellosis). The primary objective of this project was to isolate bacteriophage against Shigella dysenteriae and to recognize its different characteristics as an antimicrobial agent to determine the therapeutic potential. In this study, Shigella dysenteria specific bacteriophage was isolated and characterized to develop a therapeutic agent. A total number of 20 samples were collected for the study of this experiment. The samples were collected from different areas of Dhaka city. The samples were initially filtered and the double layer- ager method was used for the isolation of bacteriophages. For the characterization of bacteriophages, host range specificity tests were checked. After examination of the host range, the Shigella dysenteriae phage stated that it had a wide host range as it was able to lyse 5 out of 13 distinct bacterial cultures. The most prominent bacterial hosts were ETEC, EAEC, Shiga-toxin producing E.coli (STEC), Entero pathogenic E.coli (a typical) and Shigella dysenteriae were lysed by Shigella dysenteria phage. In the case of bacteriophage isolations, clear zones were observed which indicates the presence of phages. This research, therefore, adds to the increasing amount of isolated bacteriophages, especially the particular bacteriophage of Shigella dysenteriae. Studies on its biological features may provide helpful data and understanding to identify prospective therapeutic agents against infection with Shigella dysenteriae.
    Keywords
    Shigella dysenteriae; Surface waters; Biocontrol agents; Bacteriophages
     
    LC Subject Headings
    Shigella; Genetic regulation
     
    Description
    This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Microbiology, 2019.
     
    Catalogued from PDF version of thesis.
     
    Includes bibliographical references (pages 17).
    Department
    Department of Mathematics and Natural Sciences, Brac University
    Collections
    • Thesis (Bachelor of Science in Microbiology)

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