• Login
    • Library Home
    View Item 
    •   BracU IR
    • School of Data and Sciences (SDS)
    • Department of Mathematics and Natural Sciences (MNS)
    • Bachelor of Science in Microbiology
    • Thesis (Bachelor of Science in Microbiology)
    • View Item
    •   BracU IR
    • School of Data and Sciences (SDS)
    • Department of Mathematics and Natural Sciences (MNS)
    • Bachelor of Science in Microbiology
    • Thesis (Bachelor of Science in Microbiology)
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Understanding the effect of exposure of human pathogens to polluted surface water: Vibrio cholerae as a model organism

    Thumbnail
    View/Open
    13226008_MNS.pdf (2.051Mb)
    Date
    2018
    Publisher
    BRAC University
    Author
    Islam, Syeda Sumaiya
    Metadata
    Show full item record
    URI
    http://hdl.handle.net/10361/10796
    Abstract
    Urbanization and industrialization results in the fecal organisms contaminating the water-bodies which are important sources of recreational activities. This eventually increases the risk of humans getting infected during accidental ingestion or contact. The purpose of the study was to detect the presence of Vibrio cholerae in water bodies contaminated with industrial pollutants. For this purpose Hatirjheel Lake and Buriganga River, the two prominent water bodies of our country were chosen for sampling. A total of 40 samples were collected aseptically from Hatirjheel Lake and 20 samples from Buriganga River within duration of three months. Then, they were processed for isolation of culturable Vibrio cholerae. After that several biochemical tests were done for presumptive identification of isolates. For further confirmation, RT-PCR was done targeting CqsS gene. While many isolates were found to be positive with biochemical tests, after RT-PCR only 5% of samples from both water bodies gave positive result. Further, three strains from both water bodies were tested for survival potential under starvation stress. The survival patterns between the lake and river showed slight difference but both showed a gradual decrease in culturable count over the time. Next, the strains from those water bodies were checked for their susceptibility to an array of eight antibiotics. The antibiogram revealed that isolates from Hatirjheel showed complete resistance to tetracycline, ampicillin, ceftriaxone while the Buriganga isolates showed complete resistance to only ampicillin, tetracycline, nalidixic acid, co-trimethoprim and ceftriaxone. While no conclusion could be drawn on the actual presence of Vibrio cholerae in Hatirjheel or Buriganga, isolation of this organism from heavily polluted water bodies could be difficult.
    Keywords
    Human pathogens; Vibrio cholerae; Surface water
     
    LC Subject Headings
    Vibrio infections; Cholera; Vibrio cholerae
     
    Description
    This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Microbiology, 2018.
     
    Catalogued from PDF version of thesis report.
     
    Includes bibliographical references (page 38-41)
    Department
    Department of Mathematics and Natural Sciences, BRAC University
    Collections
    • Thesis (Bachelor of Science in Microbiology)

    Copyright © 2008-2019 Ayesha Abed Library, Brac University 
    Contact Us | Send Feedback
     

     

    Policy Guidelines

    • BracU Policy
    • Publisher Policy

    Browse

    All of BracU Institutional RepositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    Statistics

    View Usage Statistics

    Copyright © 2008-2019 Ayesha Abed Library, Brac University 
    Contact Us | Send Feedback