Bacteriology of diabetic foot ulcers and their antibiotic resistance ability

Abstract

This study is aimed to isolate the pathogenic bacteria from diabetic foot ulcers and observe their antibiotic susceptibility against antimicrobial agents. Seventy-five patients diagnosed with diabetic foot ulcers were investigated. Pus samples collected from patients were cultured in the selective media to confirm the identity of the isolated pathogenic bacteria for each patient. Further, an antibiotic sensitivity test was performed by following the Kirby- Bauer Disc Diffusion Susceptibility Test Protocol. One hundred and forty-one pathogenic bacteria were identified from the ulcer patients. Proteus spp. and Klebsiella pneumoniae are the most common pathogenic bacteria isolated, respectively. Most of the Gram-negative bacteria were resistant to Beta-lactamase group’s Ampicillin. Such as Klebsiella pneumoniae (97.56%), Pseudomonas aeruginosa (95.24%), and Escherichia coli (81.82%). Proteus spp. was mostly sensitive to piperacillin-tazobactam (80%). On the other hand, Gram-positive Staphylococcus aureus showed mostly sensitivity towards both vancomycin and norfloxacin (79.17%).