HPLC in the purification of antimicrobial peptides from Andrias davidianus blood sample

Abstract

The Andrias davidianus has a long history of use in traditional Chinese medicine. Its blood is regarded as a byproduct or waste of the meat industry. Although reports of the isolation of antimicrobial peptides from various sources exist, there are no records of their isolation from the blood of A. davidianus. In this study, Andricin B, an antimicrobial peptide, was extracted from A. davidianus blood using a novel technique that combines magnetic liposome adsorption with reversed-phase high-performance liquid chromatography. Further research was done on the structure, antibacterial capability, and safety of Andricin B. N-terminal sequencing was used to determine the amino acid sequence, which was discovered to be Gly-Leu-Thr-Arg-Leu-Phe-Ser Val-Ile-Lys. A clearly defined random coil conformation was proposed by CD spectra and the three-dimensional structure prediction made by bioinformatics tools. All of the bacteria tested in this study's Andricin B tests, as well as some fungi, were shown to be resistant to it. The MICs, or minimal inhibitory concentrations, ranged from 8 to 64 g ml-1. Additionally, the haemolytic testing indicated that Andricin B might be regarded as safe at the MICs. Finally, it was discovered that Andricin B prevented Staphylococcus aureus from growing in cooked A. davidianus flesh. This work demonstrates that Andricin B is a promising new antimicrobial peptide that may offer additional insights into the creation of novel medications.