Screening of hypoglycemic effect and antioxidant potential of trewia nudilfora leaf extracts

Abstract

Diabetes is one of the most alarming diseases of the current civilization, especially due to its life-long dragging condition and increasing number of patients. In the thirst of combating such circumstances there relies a need of novel drug candidates. Our study was aimed to screening of the leaf extract of Trewia nudilfora for antioxidant and hypoglycemic activities which were selected on the basis of the phytochemical observation of the chosen specimen. Ethyl acetate extract (EATL) and chloroform extract (CLTL) of the grinded leaf were prepared for running the experiments. The antioxidant study was observed in-vitro by DPPH scavenging radical in comparison to ascorbic acid as standard at 517nm. The IC50 values of the standard, EATL and CLHL extracts were 146.88, 120.06 and 117.05 respectively. From the obtained results both the extracts are considered to have antioxidant effect and EATL was found to be higher than the other extract. For hypoglycemic study, both normoglycemic and oral glucose tolerance test (OGTT) effect of EATL and CLTL were carried out in Swiss albino mice at times 0, 30, 60, and 90minutes by a glucometer after pricking the tail vein of rodents at 250 and 500mg/kg doses where we used Metformin HCl as reference drug. In normoglycemic study we have observed that glucose reduction rate was more prominent at higher dose for EATL than CLTL (EATL: 0min-7.92; 30min- 7.4; 60min- 6.12; 90min- 5.08mm/l and CLTL: 0min- 9; 30min-9; 60min- 8.9; 90min- 9mm/l). In case of OGTT, we have noticed the similar pattern for the declination rate of glucose level for both experimented extracts. The subsequent fall of glucose level indicated the dose of EATL at 500mg/kg was very close to the standard values. For both hypoglycemic tests the results were statistically significant compared to the control (p<0.05, p<0.001). We can conclude that the leaf extract of Trewia nudiflora contains active antioxidant and hypoglycemic functions with great potential for producing a revolutionary drug.