A novel vaccine designing approach through analyzing computational tools against pathogenic vibrio cholerae’s outer membrane protein of O1 strain as adjuvant

Abstract

Cholera epidemic, history denotes it as “The Blue Death'', a deoxygenated, dehydrated death of humans due to the bacterial species named Vibrio Cholerae. When the sanitation system is extremely devastated by the purity of water and lacks the knowledge of hygiene, inhabitants make themselves vulnerable to this water and airborne microbe, Vibrio Cholerae. After getting affected by Vibrio Cholerae, a person gets desiccated due to water loss and infecting intestinal lumen (small and large intestine), if it stays untreated, the death of the person might occur in a few hours. Studying this severely spreading bacteria species and the extremity of the causing disease, an “In Silico'' approach is made to prepare a vaccine against Vibrio Cholerae. Several computational servers and tools were profoundly used during the preparation and justified their results. An In Silico'' approach is made to prepare a vaccine against Vibrio Cholerae. Several computational servers and tools were profoundly used during the preparation. The antigenicity result of the selected ""Outer Membrane Protein Sequence"" is 0.5469, stated as ""Probable ANTIGEN"". A proposed vaccine was then developed using linkers to be added and tested for antigenicity using Vaxijen V2.0 and the result was 0.6203. After that, the molecular docking was done using Patchdock and achieved a score of 20884 in a 3101.90 square angstrom region.