Development of UV-Vis spectroscopic method for determining effect of solvent on solubility of Vitamin C and Vitamin E

Abstract

This study was done to observe effect of solvent on solubility of vitamins. Vitamins studied included vitamin C (water soluble) and vitamin E (fat soluble). The solvents used included acetonitrile and distilled water. The proportions of acetonitrile and water in the solvent were varied and the effects on solubility of the vitamins were determined. To determine the solubility, UV-Vis spectroscopy methods were developed. These methods were also validated. The result that found from experiment was represented on calibration curve and discussed if any error found. As multi vitamin carries both water soluble and fat soluble compound, this study was carried out to find whether they showed same effect or not. This will be useful for conducting experiments in the future and also these types of comparative study will enrich the performance of quantitative analysis.