Immuno-informatics aided -design of multi-epitope based vaccine against Nipah henipavirus Glycoprotein G.

Abstract

In the family Paramyxoviridae, the genome of Nipah henipavirus belongs. The Henipa virus is a descendant of the Hendra and Nipah viruses. The most recent Nipah henipavirus outbreak in Kerala occurred in 2018 in the Kozhikode district. Since there are no effective antiviral drugs to treat NiV illness, patients have no other therapy choices. In this study, we developed an immunoinformatics-based multi-epitope vaccine to guard against Nipah henipavirus glycoprotein G infection. Vipr's database service provided the glycoprotein G sequence in the form of a FASTA file. After that, we used several servers to choose ctl, htl, B cell epitopes, and other predictions. We found two of the CTL epitopes here (AMDEGYFAY and GIKQGDTLY) and two of the htl epitopes here (NPLVVNWRDNTVISR and VNPLVVNWRDNTVIS) show the highest binding affinity with the glycoprotein G sequence. The antigenicity of the entire protein sequence rises from 0.52 to 0.55 when all of the antigenic epitopes are included. The Z score is positive (-8.47), the GRAVY score is positive (-0.316), which suggests that the protein is hydrophilic, the confidence level is 100%, and the coverage rate is 52%, considered a good result for the development of a vaccine.