Isolation and characterization of carcinogenic chromium reducing bacillus cereus from soil of Buriganga River

Abstract

Hexavalent Chromium has mutagenic and carcinogenic property and it causes different diseases. In many industries, it is used and often thrown to environment without proper treatment. To protect the environment and human health, a bacterial strain Bacillus cereus was identified which has the ability to reduce hexavalent chromium to trivalent chromium. Sample was collected from Buriganga River. Nutrient agar media with Potassium chromate (K2CrO4) solution was used for selection of desired strain. 16s rDNA sequencing method was followed for identification of bacterial strain. Diphenyl Carbazide base bioremediation assay was performed at 25⁰C, 37⁰C, 42⁰C and pH 5.5, 7, and 8.5. Optimum temperature and pH for reduction of chromium for the bacterial strain was found 37⁰C and pH 8.5. MIC of the bacterial strain was found 37mM at K2CrO4. Bacillus cereus shows resistance to 4 antibiotics, Cefexime (CFM5), Penicillin (P10), Sulphamethoxazole (STX25), and Amoxicillin (AML10).