Method optimization for isolation of Pseudomonas bacteriophage from soil samples

Abstract

Bacteriophages as antibiotic are very useful against antibiotic resistant bacteria in the medical fields. Bacteria like pseudomonas aeruginosa are resistant to many types of antibiotics and can cause severe diseases to human and animals. In our research project the goal is to identify and isolate these pseudomonas specific bacteriophages from the environment through different methods. In this process we collected soil samples from different region of the country like boali, comilla and tongi and Isolated bacteriophages were applied on the hard agar medium along with soft agar and respective bacterial strains to observe the plaques as bacteriophages. The methods we used to isolate pseudomonas specific bacteriophage didn’t show any promising result except method four which showed a trace amount of plaque with one sample from Boali. This research may help to reduce the cost, dependency on antibiotics and to kill the bacteria those are resistant to antibiotics. This research will help the world in the long run to control the resistant bacteria from spreading to newborn to older individuals.