In vitro anti-arthritic effect of Nypa fruticans Wurmb fruit pulp extract

Abstract

Despite the discovery of many medications, such as medicinal herbs, there is still a need to offer better, safer, and more effective drug sources for rheumatoid arthritis, an autoimmune inflammatory disease. The current study aimed to evaluate Nypa fruticans Wurmb fruit pulp extract's antiarthritic potential in patients with rheumatoid arthritis. To evaluate the anti-arthritic effect of this methanolic fruit pulp extract, in vitro suppression of protein denaturation (bovine serum albumin) was performed. In this investigation, five distinct test concentrations (100, 200, 300, 400, and 500 μg/ml) were used. The observed action was shown to be dose-dependent. Using the protein denaturation method, the chosen extract at 500 μg/ml demonstrated the highest level of protection (76.28±1.05%), while the standard medication offered 96.77±0.78% protection. Our conclusion was that, in comparison to the usual medication, diclofenac sodium, NFP extract demonstrates strong anti-arthritic activity at varied concentrations. However, to verify its effectiveness and mode of action, more pharmacological research utilizing separate active components must be done.