In vitro biological screening of ethanol extract of Pterocarpus Indicus Willd. (Fabaceae) leaves

Abstract

This research was carried out to determine the biological properties (cytotoxic, antimicrobial) of a medicinal plant, named Pterocarpus Indicus Willd, which belongs to Fabaceae family. For evaluating cytotoxic property of ethanol extract of Pterocarpus Indicus Willd leaves, BrineShrimp Lethality Assay has been done. The obtained LC50 value was 1260.99μg/mL obtained for ethanol extract and for the standard Vincristin Sulfate it was 4.09 μg/mL. Additionally, In high concentration the percentage of mortality was very low and in low concentration the percentage of mortality rate was zero. So, Pterocarpus Indicus Willd do not have cytotoxic effect. For Antimicrobial property evaluation, Disk diffusion method has been done. There were high range of bacterial growth in every concentration of Bacillus subtilis, Escherichia coli, and Salmonella typhi bacteria. Only in the bacterial stain of Staphylococcus aureus, the range of bacterial growth is low in every concentration so, this plant have low level of antimicrobial effect. This research is essential to the discovery of pharmacological properties of this plant.