An in-vivo study on anti-emetic and in-vitro study on anthelmintic properties of cassia fistula

Abstract

Cassia fistula is a tropical and sub-tropical legume tree which is widely known for its antioxidant, anti-inflammaory, antitumor, hypolipidemic, antimicrobial, hepato-protective and many pharmacological activities. The present study focuses on the investigation of antiemetic and anthelmintic activity of crude methanolic extract of Cassia fistula using 2-4 days chicks (32-52gm) model and Pheretima posthuma model respectively. The chick samples were administered with copper sulphate by oral route of administration to initiate emesis among them. Afterwards they were administered with leaf extract (150 mg/kg) orally which exhibited significant antiemetic property (61.58%, 88.41% and 30.79%) compared with reference drug chlorpromazine (29.88%). In-vitro anthelmintic tests were done with earthworms (Pheretima posthuma) by using Cassia fistula leaf extract of different concentrations (60 mg/mL, 80 mg/mL and 100 mg/mL). Albendazole (20 mg/mL) was included as a standard reference and for control distilled water was used. The two concentrations of Cassia fistula extract which were 80 mg/mL and 100 mg/mL exhibited significant results which were superior than the standard used (albendazole).