In-silico based multiepitope vaccine construction of envelope glycoprotein against Hantavirus

Abstract

Hantavirus is a zoonotic virus which is responsible for causing hemorrhagic fever with renal syndrome & hantavirus pulmonary syndrome. But still, there is no stable vaccine developed for this virus. Developing a multiepitope vaccine candidate against this virus was the main concern of this study by using immune-informative approach. Different computational tools were used to identify the CTL, HTL & B cell epitopes for envelope surface glycoprotein. Various linkers were used to connect the epitopes with the adjuvant and construct the candidate vaccine. Physicochemical properties like - molecular weight, instability index, aliphatic index, GRAVY was also checked for the prepared vaccine. Molecular docking was done with toll-like receptor 4 (TLR-4) for checking the residual interaction. And lastly, C-ImmSim was used to evaluate the immune stimulation. Further investigation is needed to check the safety and efficacy of this vaccine candidate because checking safety & efficacy is not a part of in-silico study.