A novel vaccine designing method using in-silico tools against scaffold protein OPG 125 of monkeypox virus

Abstract

Recently, the number of patients with monkeypox disease is increasing at an alarming rate which has made the necessity of inventing a vaccine against this disease. As a result, this study is performed in order to design a vaccine model against the disease based on in silico computational tools. Scaffold protein OPG 125 has been finalized as the primary protein of this vaccine. CTL, HTL and B cell epitopes are filtered against their antigenic, allergic and toxic elements. The tool Vaxijen v2.0 has identified a 0.5312 antigenic score which has been increased from 0.5352. While evaluating biochemical properties, physical and chemical features were also examined. It shows a negative z score and gravy score which is required for this process. Additionally, 3D model assessment has also been carried out and it provides 80% coverage with a confidence of 100%. In the end molecular docking and immune simulation is performed by using Cluspro and C-ImmSimm.