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A study on the microbiological status of mineral drinking water
(© 2017 Bentham Science Publishers B.V., 2017-04-01) Aditi, Faria Y.; Rahman, Shafkat Shamim; Hossain, Md Manjur; Department of Mathematics and Natural Sciences
Introduction: Water-borne diseases constitute a major health burden in Bangladesh. The objective of this study was to assess the overall quality of mineral water samples that obtained from different shops of Dhaka city. Material and Methods: To achieve the above-mentioned objective, methods of heterotrophic plate count (HPC) and total coliform count (TCC) were applied. Moreover, isolated colony from mineral water samples were characterized by using biochemical and antimicrobial susceptibility tests. Results: Different water samples showed different HPC ranged from 1.0×10 to 8.00×102. Antimicrobial sensitivity test of some selected bacteria viz S. intermedius, S. aureus, S. felis and S. Saccharolyticus were performed. It was observed that Staphylococcus spp. isolates were susceptible to erythromycin, tetracycline, norfloxacin and ciprofloxacin. Furthermore, a few Staphylococcus spp. isolates were intermediate resistant to penicillin and oxacillin. However, most of the Staphylococcus spp. isolates were resistant to cefixime. Conclusion: The results indicate that mineral water serves as a reservoir of various bacteria and that people in Dhaka city, who are the consumers of these water, might get diseases. This study emphasizes the need for elaborated microbiological examinations of mineral drinking water commonly used in Dhaka city.
In vitro shoot micro propagation of medicinal applications and ornamental value of Cestrum nocturnum L.
(© 2016 Indian Society for Education and Environment, 2016) Sarkar, Mohammad Khairul Islam; Jahan, Miskat Ara Akhter; Roy, Chapol Kumar; Hossain, Khaled; Rahman, Shafkat Shamim; Mia, Md Rubel; Islam, Mohammad Rafiqul; Azim, Dewan Anwarul; Department of Mathematics and Natural Sciences
Background/Objectives: Cestrum nocturnum L. a night Blooming jasmine belongs to Solanaceae family widely circulated all over tropical as well as subtropical areas of the World. It is mainly popular for ornamental fragrant flowering and hedge plant but also sometimes for traditional medicinal purpose. Due to strongest smelling characteristics of the plants, it is used in many industries for making Perfumes, essential Oils, Soaps, Candles, Body Oils, etc. The existence of natural plants of economic importance are threatened due to rapid urban development, including industrialization, residential development, educational, commercial etc., reduce the land for cultivation. Hence plant tissue culture protocol may be adapted for production and utilization of economically popular plants, including C. nocturnum involving limited space and short period of time. Methods: Shoot tip explants of naturally grown C. nocturnum were excised sterilized and endued on 'Murashige and Skoog' (MS) medium enriched changed concentration of BA, NAA, as well as GA3 singly or in combination. Excised micro shoots were examined for root development on 0.5 MS using IBA, NAA as well as IAA separately. Findings: The highest amount of multiple bud were observed in low concentration of BA (01.50 milligram × l-1), resulted no. of shoot 4.40 as well as 4.20/explant, no. of leaves 15.40 as well as 4.20/explant as well as size of different shoot 5.360 as well as 4.860 cm. The concentration of IBA and IAA were found to be best for root formation in micro shoots (13.20, 6.80 roots/micro shoots) as well as root size (8.39, 5.73 cm) individually. Application: There are many opportunity of plant tissue culture which offer marvelous chances in plant propagation, plant development as well as creation of plants with necessary agronomical features. Finally often hardening plantlets were gradually adjusted to natural condition and acclimatized with 90% success. This established protocol could help plant cell biotechnology, horticulture, medical and industrial sector of the country.
Isolation of yeasts from raisins and Palm-juice and ethanol production in molasses medium
(© 2016 Indian Society for Education and Environment, 2016) Rahman, Shafkat Shamim; Islam, Sarkar Mohammad Khairul; Rafiqul, Islam Mohammad; Khaled, Hossain; Kamrun, Nahar; Kumar, Roy Chapol; Uddin, Md Ekhlas; Choudhury, Naiyyum; Department of Mathematics and Natural Sciences
The alternative fuels are expected to satisfy the progressive demand for energy on the wake of the negative effects of fossil fuel on the atmosphere and resultant universal warming. In this study two ethanol fermenting Saccharomyces cerevisae were isolated from Palm juice and Raisins. Method/Statistical Analysis: Both isolates were grown in Yeast extract Peptone Dextrose (YEPD) medium and characterized for alcoholic fermentation using molasses medium and optimized for pH, thermo-, osmo-, ethanol tolerance and sugar concentration. Findings: Results showed for ethanol fermentation, 31°C temperature, 6.01 pH and 6.50% sugar concentration is the prime condition. Raisin-isolate emerged as highly thermophilic and stress tolerant in nature. Under optimized conditions, S. cerevisae isolated from Palmjuice produced 9.85% of ethanol in the medium. Application/Improvements: Creation of ethanol through fermentation appears to be a potential other fossil fuel and can be used as exclusive fuel in vehicles with dedicated engines or in fuel blends.

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