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The effect of verotoxin producing escherichia coli in development of experimental gastroenteritis among streptomycin treated murine model: a longitudinal study
(© 2013 Bangladesh Journal of Microbiology, 2013-06) Mosharraf, Fahareen Binta; Ahsan, Chowdhury Rafiqul; Nessa, Jamalun; Yasmin, Mahmuda; Department of Mathematical and Natural Science
Escherichia coli O157:H7 is a newly emerged pathogen, which has been recognized as a major cause of large scale epidemics and thousands of sporadic cases of gastrointestinal illness. This observation particularly calls for the in vivo examination of E. coli O157 candidates in an attempt to mimic various aspects of E. coli O157:H7 disease symptoms in humans. Healthy conventional Swiss albino mice were used for investigating the clinical manifestation exerted by stx1A and stx2A positive E. coli O157:H7 in various doses applied through three alternative routes (oral, intramuscular and intraperitoneal). The highest titer of orally added E. coli 0157:H7 (109 CFU ml-1) among the five test doses had started symptoms at the earliest time and manifested most of the classical symptoms. The symptoms started to become visible at 14th hour, increased with time and had reached moribund condition near 48th hour just before death of the host. The oral way of E. coli O157:H7 addition at the dose of 100 μl suspension containing 1 X 109CFU ml-1 was taken as the most potent concentration in producing bacterial fatality and hence was selected as the Minimum Lethal Dose (MLD).
An appraisal of bacteriophages as biocontrol agent in treating E.coli O157:H7 mediated experimental gastroenteritis in mice
(© 2015 International Journal of Multidisciplinary Research and Development, 2015-04) Mosharraf, Fahareen-Binta-; Ahsan, Chowdhury Rafiqul; Yasmin, Mahmuda; Nessa, Jamalun; Department of Mathematical and Natural Science
The emergence of new pathogenic bacteria resistant to most, if not all, currently existing antimicrobial agents has become a serious problem in modern medicine, particularly because of the affiliated increase in immunosuppressed inhabitants worldwide. The recent surfacing of E. coli O157:H7 as a foremost food pathogen is a lively reminder of its dynamic character. Mounting concerns about drug-resistant pathogenic bacteria have rekindled interest in alternative treatments of bacterial infections. Prominent among these alternatives is phage therapy for combating supportive infections. This observation particularly calls for the in vivo examination of environmental phage candidates to treat stx1A and stx2A positive Escherichia coli O157:H7 induced experimental diarrhea. Two O157-specific lytic bacteriophages PAH6 and P2BH2 were isolated from sewage and characterized. Healthy conventional Swiss albino mice were used for investigating the O157:H7 specific diarrhea applied through three alternative routes (oral, intramuscular and intraperitoneal).The highest titer of orally added E. coli 0157:H7 was found to be fatal within 24 hrs. A single oral dose of phage strain (1X107 PFUml-1) alone or in combination was sufficient to rescue 100% of the animals from this lethal challenge in vivo. Finally, the results obtained in this study suggest that cocktail of two coliphages applied orally for treatment gastrointestinal infection have good therapeutic potential.
Exploring the best possible route of infection of verotxin producing escherichia coli in development of experimental gastroenteritis among streptomycin treated murine model
(© 2015 Journal of Bangladesh Academy of Sciences, 2015) Mosharraf, Fahareen-Binta; Ahsan, Chowdhury Rafiqul; Nessa, Jamalun; Yasmin, Mahmuda; Department of Mathematical and Natural Science
The prevalence of Escherichia coli O157:H7 induced bacteremia generate a critical problem in modern medical therapy for bacterial infections. This study sought to find out the best possible route and dose of E. coli 0157:H7 infection in experimental murine model by periodic stool and blood culture count of relevant bacterial strains. Streptomycin treated mouse model were used for investigating the clinical manifestation exerted by stx1A and stx2A positive E. coli O157:H7 with increasing doses applied through three alternative routes (oral, intramuscular and intraperitoneal). The highest titer of orally added E. coli 0157:H7 among five test doses started showing symptoms at the earliest time and reached moribund condition about 48 hours just before being dead. The oral way of E.coli O157:H7 at the dose of 100 ƒÝl suspension containing 1 „e 109CFU ml-1 was taken as the most potent concentration in producing bacterial fatality and hence was selected as the minimum lethal dose (MLD).

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