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Cloning of an antiporter gene (NHX1) from PCR amplicons into a recombination competent vector containing a constitutive promoter (caMV35S)

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dc.contributor.advisor Seraj, Dr. Zeba Islam
dc.contributor.advisor Islam, Dr. Aparna Razzaque, Samsad 2012-03-14T05:38:43Z 2012-03-14T05:38:43Z 2011 2011-10
dc.identifier.other ID 10176007
dc.description This thesis report is submitted in partial fulfillment of the requirement for the degree of Masters of Science in Biotechnology, 2011
dc.description Cataloged from PDF version of thesis report.
dc.description Includes bibliographical references (page 84-87).
dc.description.abstract The costal belt of Bangladesh is under the threat of increasing salinity and thus reducing plant productivity. Consequently, salinity has become a major concern for ensuring food security. Salt tolerance is known to be a complex trait both genetically as well as physiologically and conferring this tolerance by introducing a single gene is therefore difficult. However, over expression of the vacuolar antiporter gene, NHX1 has been reported to provide salinity tolerance to a good extent. This work was carried out to clone the coding sequence of the rice antiporter gene (applying Gateway Technology) in a binary vector (pH7WG2.0) from where this can be easily introduced into a plant genome with a highly efficient constitutive promoter (CaMV35S). In this study, NHX 1 gene was first cloned into pENTR and confirmed the insertion through PCR, Restriction Digestion and Sequencing technique. Then the gene of interest was recombined from pENTR to the Destination vector (pH7WG2.0) and recombination was confirmed by PCR and Restriction Digestion respectively. Finally, recombinant vector was then transformed into Agrobacterium (the transformation was also confirmed by PCR and Restriction Digestion) to perform Agrobacterium mediated transformation into tomato plant. en_US
dc.description.statementofresponsibility Samsad Razzaque
dc.format.extent 87 pages
dc.language.iso en en_US
dc.publisher BRAC University en_US
dc.rights BRAC University thesis are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission.
dc.subject Biotechnology
dc.title Cloning of an antiporter gene (NHX1) from PCR amplicons into a recombination competent vector containing a constitutive promoter (caMV35S) en_US
dc.type Thesis en_US
dc.contributor.department Department of Mathematical and Natural Science, BRAC University M. Biotechnology

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