BRAC University Institutional Repository

Isolation, identification and characterization of Jute Genomic LTR retrotransposon sequences

DSpace/Manakin Repository

Show simple item record

dc.contributor.author Siddiqi, Sadaf Saaz
dc.date.accessioned 2012-03-04T09:43:08Z
dc.date.available 2012-03-04T09:43:08Z
dc.date.issued 2012-01
dc.identifier.other ID 07076003
dc.identifier.other ID 07176003
dc.identifier.uri http://hdl.handle.net/10361/1643
dc.description This dissertation is submitted in a partial fulfillment of the requirements for the degree of Masters of Science in Biotechnology. en_US
dc.description.abstract Long Terminal Repeat (LTR) retrotransposons make up a significant part of eukaryotic genomes and play an important role in plant genome evolution. Jute is an important fiber crop, and the Jute genome, consisting of 1,250 Mbps, is largely unexplored. In this study an attempt was made to identify and characterize the LTR retrotransposons of the Jute genome Chorchoru solitorius in order to understand the genome better. The Reverse Trancriptase domain of Ty l -copia and Ty3-gypsy LTR retrotransposons of Jute were amplified by degenerate primers. The sequences were analyzed and clustered into groups. Reverse transcriptase PCR was used to analyze any transcriptional activity. Sequence analysis showed a higher heterogeneity among Tyl-copia retrotransposons than Ty3-gypsy retrotransposons. One of the groups of retrotransposon Tyl-copia was found to be transcriptionally active. As the LTR retrotransposons constitute a large portion of the Jute genome, the findings of this study suggest the importance of these elements in Jute genome evolution. en_US
dc.language.iso en en_US
dc.publisher Department of Mathematics and Natural Sciences (MNS), BRAC University en_US
dc.title Isolation, identification and characterization of Jute Genomic LTR retrotransposon sequences en_US
dc.type Thesis en_US


Files in this item

Files Size Format View
Sadaf saaz Siddiqi.PDF 16.90Mb PDF View/Open or Preview

This item appears in the following Collection(s)

Show simple item record

Policy Guidelines

Search DSpace


Browse

My Account