Detection and genotype of Rotavirus a from wastewater of Dhaka and Cox’s Bazar city

Abstract

Around the world, Rotavirus (RV) is a prominent factor in severe diarrhea. This study investigated the diversity of RV genotypes in the wastewater of several defined locations that reflect the holistic comprehension of two concerned cities- Dhaka and Cox's Bazar. Our procedures comprised of RNA extraction, real-time one-step RT-qPCR, Sanger Sequencing and genomic analysis to define RV strains. Our findings showed the highest frequency of Wa-like Rotavirus strains throughout the research areas. However, a unique sequence, G2P[4] was discovered solely in the Cox's Bazar region. Notably, there were regional differences in genotypic dominance, with G1 (53%; 8/15) prevailing in Dhaka and G3 (72%; 10/14) in Cox's Bazar. While investigating the merged genotypes, G1P[8] (44%; 4/9) had higher incidence in Dhaka whereas G3P[8] (80%; 4/5) was prevalent in Cox's Bazar. By highlighting the need of wastewater surveillance in combating future outbreaks, this study can advance our understanding of RV in broad geographic contexts