HPLC in the purification of antimicrobial peptides from Andrias davidianus blood sample
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Date
2022-10Publisher
Brac UniversityAuthor
Khan, Abrar KabirMetadata
Show full item recordAbstract
The Andrias davidianus has a long history of use in traditional Chinese medicine. Its blood is
regarded as a byproduct or waste of the meat industry. Although reports of the isolation of
antimicrobial peptides from various sources exist, there are no records of their isolation from the
blood of A. davidianus. In this study, Andricin B, an antimicrobial peptide, was extracted from A.
davidianus blood using a novel technique that combines magnetic liposome adsorption with
reversed-phase high-performance liquid chromatography. Further research was done on the
structure, antibacterial capability, and safety of Andricin B. N-terminal sequencing was used to
determine the amino acid sequence, which was discovered to be Gly-Leu-Thr-Arg-Leu-Phe-Ser Val-Ile-Lys. A clearly defined random coil conformation was proposed by CD spectra and the
three-dimensional structure prediction made by bioinformatics tools. All of the bacteria tested in
this study's Andricin B tests, as well as some fungi, were shown to be resistant to it. The MICs, or
minimal inhibitory concentrations, ranged from 8 to 64 g ml-1. Additionally, the haemolytic
testing indicated that Andricin B might be regarded as safe at the MICs. Finally, it was discovered
that Andricin B prevented Staphylococcus aureus from growing in cooked A. davidianus flesh.
This work demonstrates that Andricin B is a promising new antimicrobial peptide that may offer
additional insights into the creation of novel medications.