Bachelor of Science in Biotechnologyhttp://hdl.handle.net/10361/30502024-03-28T17:02:26Z2024-03-28T17:02:26ZIsolation and Characterization of E. coli Bacteriophage from Raw Meat and Environment WaterIslam, MahbubaSiddik, Md Abu BakarMondol, SudorshonHossain, Md Shahriarhttp://hdl.handle.net/10361/224822024-03-13T03:03:13Z2023-12-01T00:00:00ZIsolation and Characterization of E. coli Bacteriophage from Raw Meat and Environment Water
Islam, Mahbuba; Siddik, Md Abu Bakar; Mondol, Sudorshon; Hossain, Md Shahriar
Several diseases such as stomach cramps, urinary tract infections, and diarrhea are caused by the bacterium Escherichia coli. One of the five subtypes of E. coli which are ETEC, EPEC, EAEC, EHEC/STEC, and EIEC are responsible for intestinal infections. E. coli outbreak is a major public health concern. One of the key elements that can put an end to outbreaks could be bacteriophages. Since their discovery in 1915, they have been beneficial to humans in a variety of ways, including phage therapy, genetic screening tools, diagnostic weaponry, pathogenic bacteria detectors, therapeutic agents, and more. Phage research has gained attention because of the recent rise in antibiotic resistance because of their capacity to infect and destroy bacteria without endangering humans. This experiment is designed to isolate and characterize bacteriophage specific to E. coli. It includes procedures that have been used in the lab to check for the presence of phages and the results of the experiments. In this research, E. coli bacteria and bacteriophage was isolated from the Raw meat. Unfortunately, only one bacteriophage was possible to isolate from Raw meat and which is why water samples are collected from various regions of Mohakhali, Gulshan and Hatirjheel and from the samples, four more bacteriophages were successfully isolated. Phage ECPW4.4, ECPW5.1 and ECPW12.3 were chosen for further analysis and characterization. In characterization, three tests were done, which were; Temperature test, salinity test and pH test. On these three tests, the three bacteriophages showed better results overall. Furthermore, more tests and analysis are needed to be done but because of the closure of BRAC University Lab, further research was not possible.
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology and Bachelor of Science in Microbiology, 2023.; Catalogued from PDF version of thesis.; Includes bibliographical references (pages 62-65).
2023-12-01T00:00:00ZIn vitro collateral sensitivity: a potential approach against multiple antibiotic resistant bacteriaYousha, Nasrat AhmedBadhan, Sadia IslamDina, Shatabdi DebAhsan, MD Jahidhttp://hdl.handle.net/10361/221492024-01-15T21:02:53Z2023-07-01T00:00:00ZIn vitro collateral sensitivity: a potential approach against multiple antibiotic resistant bacteria
Yousha, Nasrat Ahmed; Badhan, Sadia Islam; Dina, Shatabdi Deb; Ahsan, MD Jahid
Antimicrobial Resistance (AMR) has long been recognized as a major issue. With the
emergence and rapid spread of antibiotic-resistant bacteria, the world's stock of viable
antibiotics is rapidly depleting. In this regard, Collateral Sensitivity (CS) is a promising and
alternative approach with the potential to treat bacterial infections. The project aimed to study
the collateral sensitivity of gram-negative resistant bacteria by exposing clinical isolates to
antibiotics in individual and combination antibiotic trials. The results were promising for both
Klebsiella pneumoniae and Pseudomonas aeruginosa. The efficiency of Linezolid against
gram-negative bacteria was one of novel findings of this study. Exposure to Linezolid in
combination with other antibiotics produced encouraging results. 50% of the P. aureginosa
strains showed susceptibility to Ceftriaxone, Levofloxacin, Tetracycline, Amikacin and
Meropenem when exposed to Linezolid itself whereas 75% of the K. pneumoniae strains
showed susceptibility to Tetracycline. Although the clinical implementation is still under
study, the collateral sensitivity treatment could prove be an adequate addition to the current
treatment to lower the antibiotic resistance ratio. Further research at the molecular level is
suggested to better understand the effect of collateral sensitivity.
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2023.; Catalogued from PDF version of thesis.; Includes bibliographical references (pages 62-68).
2023-07-01T00:00:00ZCharacterization of bacteria isolated from fermented food and evaluation of their potential to be probioticArchi, Shucheta NaziaTabassum, NafisaJuhana, Juairiah Bakthhttp://hdl.handle.net/10361/221482024-01-15T21:02:51Z2023-08-01T00:00:00ZCharacterization of bacteria isolated from fermented food and evaluation of their potential to be probiotic
Archi, Shucheta Nazia; Tabassum, Nafisa; Juhana, Juairiah Bakth
This study aimed to isolate and biochemically characterize bacteria that are not exclusively LABs from popular fermented food products and evaluate their potential to be probiotics. As primary screening, isolated bacterial colonies were tested for antagonism against seven laboratory stains (LS) of enteric or infectious bacteria using three methods: spot-on-lawn with direct bacterial colonies, disk diffusion and well diffusion with cell-free supernatant (CFS). Biochemical tests, morphological observation and growth on selective media were used to characterize the potential antagonistic bacteria and their survivability in GI-tract conditions was determined by culturing in different media modified with bile salts and acid. Antibiotic susceptibility tests in the presence of ten different antibiotics were performed to study their resistance or susceptibility. Their potential for pathogenicity was tested via hemolysis test on blood agar and DNase test on DNase agar. Although a total of eight isolates showed signs of antagonism via a competitive advantage and/or inhibition of some of the LSs, most of them tested positive for hemolytic activity deeming them potentially harmful. Two of the bacteria that were isolated from yogurt showed no apparent sign of pathogenicity and survived well in high concentrations of bile salts as well as acidic pH. They were presumed to be Pediococcus and Paenibacillus type of bacteria which had shown antagonism against Shigella flexneri and Salmonella typhi respectively. Overall, further molecular-level research is necessary to understand the true identity and probiotic potential of the isolated bacteria.
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2023.; Catalogued from PDF version of thesis.; Includes bibliographical references (pages 53-56).
2023-08-01T00:00:00ZUnlocking the therapeutic potential of RNA: a comprehensive review of RNA-based therapyAhmad, Rafid UddinPathak, Soumyahttp://hdl.handle.net/10361/221472024-01-15T21:02:50Z2023-08-01T00:00:00ZUnlocking the therapeutic potential of RNA: a comprehensive review of RNA-based therapy
Ahmad, Rafid Uddin; Pathak, Soumya
RNA-based therapy has emerged as a promising area of study in the field of medicine, presenting new therapeutic possibilities and a wide array of applications. This comprehensive review aims to examine the diverse landscape of RNA-based therapy, encompassing various types of RNA based therapies, their translation into clinical applications, and potential future developments. The article initiates with an introductory section that explores the importance and potential of therapeutic approaches based on RNA. The text subsequently classifies different forms of RNA based therapy, encompassing small interfering RNAs (siRNAs), messenger RNAs (mRNAs), antisense oligonucleotides (ASOs), and additional variants. Furthermore, the present review examines the pivotal significance of delivery systems, including strategies based on nano particles and other novel approaches, in efficiently conveying RNA therapeutics to their intended destinations. Within the context of the expanding potential, the aforementioned article also sheds light on the obstacles faced in the realm of RNA-based therapy, including impediments related to delivery and unintended effects on non-targeted areas. This statement highlights the importance of pre-clinical research and development, which plays a critical role in enhancing safety and effectiveness prior to advancing to clinical trials and eventual commercialization. Moreover, this review critically evaluates the utilization of RNA-based therapy across diverse pathological conditions, encompassing its prospects in genetic manipulation and its role in combating the COVID-19 pandemic. The scope of this discussion encompasses personalized medicine, with a focus on the exploration of RNA-based therapies and their potential for customization to suit the specific needs of individual patients, thereby leading to improved treatment outcomes. Simultaneously, the ethical and social ramifications are taken into account, emphasizing the significance of conscientious and fair utilization of these advanced therapies.
This article examines prospective avenues in the domain of RNA-based therapy, contemplating forthcoming progress and innovations within this dynamic and rapidly developing discipline. Additionally, this study examines the regulatory framework that governs RNA-based therapy and explores the intricate landscape of intellectual property rights. In addition to conducting research and development, the review critically examines the obstacles and progress in the realm of large
scale manufacturing and the clinical application of RNA-based therapies. This study explores the potential utilization of RNA-based therapy in the context of rare diseases and its implications for healthcare systems in developing nations. This review presents a comprehensive exploration of the interactions between RNA-based therapy and the micro biome, thereby broadening the scope of potential therapeutic interventions and offering intriguing prospects. In summary, this comprehensive review offers a comprehensive understanding of RNA-based therapy, providing detailed insights into its various applications, challenges, and future prospects. This statement highlights the considerable potential of RNA-based therapies to significantly transform the field of healthcare and establish new approaches to treatment in the foreseeable future.
This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2023.; Catalogued from PDF version of thesis.; Includes bibliographical references (pages 74-86).
2023-08-01T00:00:00Z