Ahmed, AkashSalsabil, Parsha2024-05-072024-05-07©20232023-08ID 18226002http://hdl.handle.net/10361/22755This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Pharmacy, 2023.Cataloged from PDF version of thesis.Includes bibliographical references (pages 65-71).Around the world, Rotavirus (RV) is a prominent factor in severe diarrhea. This study investigated the diversity of RV genotypes in the wastewater of several defined locations that reflect the holistic comprehension of two concerned cities- Dhaka and Cox's Bazar. Our procedures comprised of RNA extraction, real-time one-step RT-qPCR, Sanger Sequencing and genomic analysis to define RV strains. Our findings showed the highest frequency of Wa-like Rotavirus strains throughout the research areas. However, a unique sequence, G2P[4] was discovered solely in the Cox's Bazar region. Notably, there were regional differences in genotypic dominance, with G1 (53%; 8/15) prevailing in Dhaka and G3 (72%; 10/14) in Cox's Bazar. While investigating the merged genotypes, G1P[8] (44%; 4/9) had higher incidence in Dhaka whereas G3P[8] (80%; 4/5) was prevalent in Cox's Bazar. By highlighting the need of wastewater surveillance in combating future outbreaks, this study can advance our understanding of RV in broad geographic contexts71 pagesenBrac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission.GenotypeRotavirusWastewaterRotavirus infectionsWater--SamplingSewage--SamplingThesis