Ahmed, AkashIslam, Tanha HasinFerdous, Zahan2023-04-102023-04-1020222022ID: 18136023ID: 18136016http://hdl.handle.net/10361/18126This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology 2022.Catalogued from PDF version of thesis.Includes bibliographical references (pages 42-47).Bacterial cellulases nowadays have been gaining considerable attention due to its enormous use in biotechnology. The study here was pursued with the aim to isolate cellulolytic bacterial strains from kitchen wastes i.e. papaya peel, banana peel and cucumber peel. Here the kitchen wastes were chosen as sources because these were easily accessible and cost-effective. And also cellulases extracted from these sources could be an effective alternative to the conventionally used hazardous chemicals. Based on DNS Assay, the isolates with maximum cellulolytic activity were selected for optimization of culture condition using two physical parameters; pH and temperature. Among the selected isolates, Bacillus subtilis (0.5 U/ml) and Bacillus cereus (0.42 U/ml) were found to be the best cellulase producers at pH 5.0 and temperature 37o C. These obtained cellulase producing bacteria could be used in industrial scale if further studied.47 pagesenBrac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission.Kitchen wasteCellulose-degrading bacteriaCarboxymethylcelluloseEnzymatic ActivityBiochemical TestFood--Composition.Cellulase.Thesis