Ahmed, AkashMahmud, HasibTushar, Jahid HasanHaque, Sanjeda2023-05-152023-05-1520232023-02ID 18326018ID 18326021ID 18126054http://hdl.handle.net/10361/18285This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Microbiology, 2023.Catalogued from PDF version of thesis.Includes bibliographical references (pages 59-65).Staphylococcus aureus is Gram-positive cocci, facultatively aerobic that has the intrinsic ability to ferment carbohydrates and forms clusters. Staphylococcus aureus is responsible for numerous pyogenic infections, food poisoning, and toxic shock syndrome, and it can produce a wide range of virulence factors. S. aureus strains that are resistant to virtually all antibiotics, with the exception of Vancomycin, have emerged in recent years. Hospital wastewater has a direct influential role in the spread of infectious diseases in healthcare settings, community settings, hospital employees, and the environment. HWW is a significant source of ARGs and ARB, and its infectious and toxic characteristics make it extremely hazardous. A total of 70 samples were collected from our study sampling sites in several phases from the period of June 2022 to December 2022. From the 70 samples, 21 PCR-confirmed staphylococcus aureus isolates were obtained which was 30% of the sample size. It was explored that 100% of the isolates from hospital effluents were significantly resistant to 9 antibiotics (Amoxicillin, Levofloxacin, Cloxacillin, Ceftriaxone, Ceftazidime, Erythromycin, Cefuroxime, Oxacillin, and Vancomycin), whereas 100% of the isolates from communities tap water showed resistance to 4 antibiotics (Amoxicillin, Cloxacillin, Erythromycin, and Oxacillin). The result of our study showed the emergence of ARGs in the strains of Staphylococcus aureus in the community setting has increased significantly. These ARBs and ARGs were hypothesized to be transmitted from the hospital settings by the hospital’s untreated effluents.65 pagesenBrac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission.Staphylococcus aureusARGsARBsMRSAMulti-drug resistantHospital wastewaterStaphylococcus aureus infectionsPathogenic bacteriaMicrobiologyThesis