Hossain, MahboobSaiyan, Zwad Al2026-05-072026-05-0720262026-02ID 23276007http://hdl.handle.net/10361/28225This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2026.Catalogued from PDF version of thesis.Includes bibliographical references (pages 20-23).Vitamin D deficiency is highly prevalent in South Asia despite abundant sunlight, and outdoor workers are often assumed to be protected due to prolonged sun exposure. This cross-sectional study assessed vitamin D status and associated biochemical parameters among 152 male rickshaw pullers in Dhaka, Bangladesh. Data were collected through interviews, anthropometric measurements, and biochemical analyses. Serum 25-hydroxyvitamin D was measured using chemiluminescence immunoassay. Vitamin D insufficiency was observed in 43.4% of participants, while 56.6% had sufficient levels; no participants were deficient. The mean serum vitamin D concentration was 31.39 ± 7.61 ng/mL. Vitamin D levels were not significantly associated with socio-demographic, occupational, or lifestyle factors. Dyslipidemia was common, with elevated triglycerides and total cholesterol observed in 36.8% and 34.2% of participants, respectively. Smoking was significantly associated with higher triglyceride levels (p = 0.004). Regarding electrolytes, potassium levels were within the normal range for all participants. However, calcium abnormalities were frequent, with 40.8% below and 23.7% above the normal range, while mild deviations were also observed for chloride and CO₂ levels. Despite extensive sun exposure, vitamin D insufficiency remains prevalent, highlighting the need for integrated occupational health interventions.32 pagesenBRAC University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission.Vitamin D statusRickshaw pullersOutdoor workersDyslipidemiaVitamin D deficiency.Urban health--Bangladesh.Thesis