Siam, Mohammad Kawsar SharifNabin, Md. Naimur Rahman2024-07-152024-07-15©20242024-02ID 19346041http://hdl.handle.net/10361/23697Cataloged from the PDF version of thesis.Includes bibliographical references (pages 55-60).This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Pharmacy, 2024.Cytomegalovirus is a virus causing irreversible neurological damage in newborns. In this study, in silico computational method was performed where, as primary protein, Capsid Vertex Component 1 Protein of cytomegalovirus from Uniprot server was selected. Using Vaxijen v2.0 it’s antigenicity (0.5142) & Cytotoxic T lymphocyte,helper T lymphocyte,B cell epitopes was found via NetCTL-1.2, NetMHCIIpan 4.0, Bepipred servers. Helper T lymphocyte epitopes were Screened via IFN, IL-4Pred & IL-10Pred epitopes. For constructing the vaccine, linkers were used. A Biochemical analysis gave potential results. With ProtParam the instability index (34.10), grand average of hydropathy (-0.263) & molecular weight (56399.91 kDa) was predicted. Using AllergenOnline & T3DB, the vaccine was discerned as non-allergen & non-toxic. ProSAweb gave Z-score (-5.95) & SWISS-MODEL generated Ramachandran plots. By C-IMMSIM, desirable responses of immune cells & antibodies in accordance with three doses of the vaccine was analyzed. In vitro, in vivo methods can be done to validate the safety & efficacy of the vaccine.74 pagesenBrac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission.CytomegalovirusMulti-epitope vaccineCapsid vertex component 1Biochemical analysisCytomegalovirus infections--TreatmentThesis