Hossain, MahboobMuhammad, NazifTabib, Hasan MostafizSaha, Prome2025-10-072025-10-0720252025-09ID 21236001ID 21236043ID 19336004http://hdl.handle.net/10361/26830This thesis is submitted in partial fulfillment of the requirements for the degree of Bachelor of Science in Biotechnology, 2025.Catalogued from PDF version of thesis.Includes bibliographical references (pages 40-43).Lead pollution in industrial wastewater poses an important environmental and health crisis due to its toxicity and persistence. To address this issue, two soil samples were collected from two lead-contaminated industrial zones in Bangladesh, an automobile workshop situated in Mohakhali, Dhaka and an old battery factory area in Comilla. Atomic Absorption Spectroscopy confirmed elevated levels of lead (Pb) (42.6 ppm and 61.5 ppm respectively). Metagenomic analysis of soil samples by bacterial 16s rDNA sequencing revealed diverse microbial populations, dominated by Pseudomonadati and Bacillati phylum, suggesting adaptation to heavy metal stress in natural settings. AAS of the cell free supernatants from isolated bacteria demonstrated remarkable bioremediation efficiency, removing approximately 97% and 95% of lead from 100 ppm lead-supplemented solution. Further analysis by 16S rDNA sequencing of the isolated bacterial strain L1 and L2, revealed morphological adaptations under lead stress and identified both isolates as Priestia spp.43 pagesenBRAC University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission.Lead contaminationIndustrial wastewaterLead resistanceBioremediationHeavy metal stressSoil bacteriaHealth crisisToxicityLead poisoning--Bangladesh.Factory and trade waste--Management.Sewage--Purification.Soil microbiology.Heavy metals.Bioremediation.Thesis