Naser, Iftekhar Bin2024-12-022024-12-0220222022-09ID 21176003http://hdl.handle.net/10361/24846This thesis report is submitted in partial fulfillment of the requirement for the degree of Master of Science in Biotechnology, 2022.Cataloged from PDF version of thesis.Includes bibliographical references (pages 48-56).Microorganisms isolated from soil samples that showed growth on carboxymethylcellulose (CMC) agar media were selected for enzyme production by utilizing carbon sources such as CMC, leaves and paper. Biochemical analysis results indicated a possibility of the isolates being Pseudomonas fluorescens biovar and Streptococcus sp. Cellulase produced from the symbiotic effect of the obtained microorganisms was found to be exponentially more potent than the enzymes produced by them separately. The enzyme activity on using CMC as carbon source was noted as 0.55 U/m. The enzyme activity obtained from using leaves and paper as carbon source was slightly less. The enzyme activity of the purified protein was found to show an seven-fold increase. The purified protein showed good activity within a pH range of 2-12, with an optimum ph of 6.5. The enzyme activity was maintained with negligible variation within a temperature range of 4°C to 43°C. High salinity did not affect the efficiency of the enzyme either. The enzyme seems to possess good potential for use in the paper, textile and medicine industry.56 pagesenBrac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission.Carboxymethylcellulose (CMC) AgarCellulolytic bacteriaCellulaseEnzymatic activityProtein purificationSynergistic effectThesis