Hossain, M. MahboobZaman, Ifthikhar2024-09-252024-09-25©20242024-04ID 23376006http://hdl.handle.net/10361/24191This thesis is submitted in partial fulfillment of the requirement for the degree of Master of Science in Biotechnology, 2024.Cataloged from PDF version of thesis.Includes bibliographical references (pages 69-83).Plastic pollution has become a major environmental concern globally, and novel and eco-friendly approaches like bioremediation are essential to mitigate the impact. This study investigated the biodegradation of three common plastic types, LDPE, LLDPE, and EPS, by Zophobas atratus larvae. Over 36 days, the average larval consumption was found to be 24.04% LDPE, 20.01% EPS and 15.12% LLDPE. FTIR analysis confirmed plastic oxidation in the gut. Gut bacteria were selectively isolated and identified as Pseudomonas aeruginosa strains. These bacteria showed the ability to degrade specific plastic types confirmed by SEM. Whole genome sequencing revealed many enzymes, along with virulence factors, antibiotic-resistance genes, and rhamnolipid biosurfactant biosynthesis genes in both isolates. Rhamnolipid analysis and AST were performed. This study indicated Zophobas atratus larvae as potential LDPE, LLDPE, and EPS biodegradation agents. Additionally, the isolated strains of Pseudomonas aeruginosa provide a more direct and eco-friendly solution for plastic degradation.96 pagesenBrac University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission.Zophobas atratusPlastic pollutionLinear low-density polyethyleneExpanded polystyrenePseudomonas aeruginosaPlastic scrap--Environmental aspects.Microbial ecology.Bacteria.Thesis