Evaluation of antibacterial, antioxidant and toxicological activity of crude extracts of Adiantum capillus-veneris, Blumea lacera, Cassia alata, and Cissus quadrangularis from Faridpur, Bangladesh

Abstract

Antibacterial and antioxidant potentials of Adiantum capillus-veneris, Blumea lacera, Cassia alata, and Cissus quadrangularis from Faridpur, Bangladesh was investigated in this study along with their toxicological evaluation. Plant materials were extracted with organic solvents (chloroform and methanol) using Soxhlet extractor. Inhibitory action of methanol and chloroform extracts of selected plants against three Gram-positive clinical isolates ¬Staphylococcus aureus, Bacillus subtilis, Bacillus cereus and three Gram-negative clinical isolates - Escherichia coil, Vibrio cholerae, Salmonella typhi was studied using agar disk diffusion, agar well diffusion methods. Broth microdilution method was utilized against Escherichia cols 0157:H7 strain and clinical isolate of Staphylococcus aureus to define the minimum inhibitory concentration of methanol extracts of selected plants. Antioxidant potential of methanol extracts of selected plant was studied using DPPH free radical scavenging assay. Morphological study of methanol extract treated distal kidney (MOCK) cell line revealed the toxicological susceptibility. Investigated plants showed no antibacterial activity against the tested clinical isolates. Significant antioxidant activity was demonstrated in the methanol extracts of investigated plants. Only Cissus quadrangularis was devoid of toxic action in the cytotoxicity assay against MDCK cell line.