Using synergic relation between bacteriophage and a bacterial species for checking the effects of antibiotic resistance genes on the lytic property of bacteriophages.

Abstract

Bacterial transformation is a process of horizontal gene transfer (hgt) where a few bacteria pick up foreign genetic material from the environment. In terms of newer therapies being applied, antimicrobial resistance is getting worse. One may expect to see a return to the pre-antibiotic age of clinical treatment if discoveries slow down. Bacteriophage or phage virus is the only entity that can infect and kill harmful bacteria. However, after a century of predominant phage therapy, the public has remained unguided and ineffective in applying this technology to treating bacterial diseases. In this study, WE want to see whether the acquisition of antibiotic resistance genes has any effect on the sensitivity of the bacteria to particular bacteriophages. For this, IN some strains specific antibiotic-resistant genes were introduced, followed by plaque assay of bacteriophage.