Isolation, molecular characterization and antibiotic resistant of pathogenic bacteria obtained from raw meat in Dhaka city

Abstract

A large number of people intake proteins from meat. But the problem is a number of bacteria are being identified during the several years from raw beef which cause different physical problems and sometimes turns into life-threatening. Improper handling and poor hygiene while handling and preparing meat causes different kinds of health problems in Bangladesh. Transmission of E. coli through the contact with animals and individual to individual via the fecal-oral route has become common. In this study, 24 samples of raw beef were collected from different areas of Dhaka city. After performing biochemical tests Eschrerichia coli, Staphylococcus, Bacillus, and Enterobacteriaceae were found in the selective media also. In this research biochemical tests such as streaking on EMB, Macconkey, XLD, and Nutrient agar medium were done. After that, antibiotic sensitivity testing was done by using 4 antibiotics; they are Kanamycin, Gentamycin, Erythromycin, and Streptomycin. Resistance was found against Erythromycin from 7 samples. Genomic DNA was isolated from samples. Then PCR was done with 16SrRNA and STX 1 separately to identify the presence of STX 1 and STX 2. Gel electrophoresis results showed bands which correspond to the presence of 16SrRNA and STX 1,that signifies the presence of pathogenic bacteria in meat. DNA sequencing of 2 samples showed that they are Enterobacter and Lactobacillus brevis.