Analysis of antimicrobial resistance in acinetobacter baumannii and klebsiella pneumoniae from patients with wound infections

Abstract

This study assessed the antimicrobial resistance profiles, key resistance genes, and hemolytic phenotypes of the organisms Acinetobacter baumannii and Klebsiella pneumoniae isolated from 61 pre-surgical traumatic leg wound samples in Dhaka. Of the specimens, 34 (55.7%) yielded target organisms: 15 A. baumannii and 19 K. pneumoniae. Both species demonstrated extremely high multidrug non-susceptibility across all tested antibiotic classes, with A. baumannii showing complete resistance to several agents. Colistin and Tigecycline remained the only antibiotics with comparatively lower resistance. All isolates exhibited γ-hemolysis, indicating the absence of RBC lysis. Molecular testing confirmed the presence of class 1 and class 2 integron integrase genes in A. baumannii, supporting the observed co-resistance patterns. These findings underscore a severe local resistance burden that compromises empiric therapy, highlight the need for routine culture-guided management, and emphasize the importance of strengthened antimicrobial stewardship and infection-control measures in trauma care settings.