Industrially beneficial microbes from kitchen waste: A green approach to produce Cellulase Enzyme and optimization of culture condition

Abstract

Bacterial cellulases nowadays have been gaining considerable attention due to its enormous use in biotechnology. The study here was pursued with the aim to isolate cellulolytic bacterial strains from kitchen wastes i.e. papaya peel, banana peel and cucumber peel. Here the kitchen wastes were chosen as sources because these were easily accessible and cost-effective. And also cellulases extracted from these sources could be an effective alternative to the conventionally used hazardous chemicals. Based on DNS Assay, the isolates with maximum cellulolytic activity were selected for optimization of culture condition using two physical parameters; pH and temperature. Among the selected isolates, Bacillus subtilis (0.5 U/ml) and Bacillus cereus (0.42 U/ml) were found to be the best cellulase producers at pH 5.0 and temperature 37o C. These obtained cellulase producing bacteria could be used in industrial scale if further studied.