Genotyping of a Clinically Important SNP rs1799853 Present in the CYP2C9 Gene Using Tetra-primer ARMS PCR Method

Abstract

Pharmacogenetics is the study of how similar drugs affect different people differently according to their unique genetic makeup as an individual. Pharmacogenetics includes a wide variety of research and discovery including essential medication disclosure, genetic research of pharmacokinetics as well as pharmacodynamics, new medication improvement and persistent hereditary testing where the final objective is to find out how a person’s gene is responding to different medications and find the best possible treatment for that individual or that group of individuals. By foreseeing the medication reaction of an individual, it will be conceivable to build the accomplishment of treatments and decrease the rate of unfavorable symptoms.In this research, our primary intent is to develop a SNP genotyping method for a particular drug, which is a sulfonylureas drug in the case of type 1 diabetes, and how it affects the functionality of CYP2C9 gene in human body. Finally, our goal is to identify whether our desired SNP is present in the particular locus using the Tetra primer ARMS PCR technique and design the drug dose of that patient accordingly