PCR identification of Pseudomonas aeruginosa and Klebsiella pneumoniae Isolated from drinking water provided in tea stalls and road-side restaurants of Dhaka North City Corporation (DNCC) and their antibiotic resistance pattern

Abstract

This study aimed to identify two specific bacterial species, Klebsiella pneumoniae and Pseudomonas aeruginosa, in the drinking water available at food stalls and roadside restaurants in Dhaka North City Corporation (DNCC) and analyze their antibiotic resistance patterns. A total of 16 water samples were collected from six different locations, with at least two samples from each location. The water samples were enriched in LB and spread plated on KPC agar media to screen for suspected K. pneumoniae isolates and on Cetrimide agar for P. aeruginosa. DNA was extracted from each isolate, and PCR amplification was performed using specific primers for molecular identification. Out of 321 isolates, 180 were confirmed by PCR amplification. Subsequently, the antimicrobial resistance of these isolates was assessed using 12 antibiotics. Alarmingly, all 90 Pseudomonas aeruginosa isolates exhibited resistance to Amoxicillin, Cefixime, and Amoxiclav. Significant resistance was also observed for Kanamycin (71.1%) and Tetracyclines (25.5%). Among the Klebsiella pneumoniae isolates, all 90 strains were completely resistant to Amoxicillin. Furthermore, these bacteria displayed varying degrees of resistance to Cefixime (82.2%), Colistin (48.8%), Azithromycin (30%), Ciprofloxacin (15.5%), and Ceftriaxone (6.6%).