Comparative study of the presence of stx1 and stx2 genes from clinical and environmental samples of Shigella

Abstract

Shigellosis, a disease caused by Shigella spp. occur because of the presence of stx1 and stx2 genes. Shigellaspp. Sometimes escapes detection by the traditional culture methods. Bacterial isolates were chosen on the basis of their structural, morphological and biochemical character. To get the most efficient result along with traditional method PCR technique was used. Antibiotic sensitivity testing was done to see the resistance of the isolates against Amoxicillin, Ampicillin, Tetracyclin, Chloramphenicol, Norfloxacinantibiotic.To detect stx1, Ka1F and Ka1R primers were used which yielded 348 bp fragment of stx1 gene. For stx2 gene Ka2F and Ka2R primers were used to get 584 bp fragment of stx2 gene. The thermal profile for 35 cycles were set using the following parameters 94°C for 10 minutes,94°C for 1 minute,55°C for 1 minute,72°C for 1 minute and 72°C for 7 minutes. This study was conducted to see the presence of Shigella spp. on the environment. Also, to find out if the environmental Shigella spp. has stx1 and stx2 gene required to cause disease.