In vitro antibacterial activity of ethanol, methanol and water extracts of persimmon fruit (diospyros peregrine) against some multi-drug resistant human pathogens

Abstract

Diospyros peregrine is a small middle sized tree of costal Bangladesh. The fruits have ethno medicinal significance for the treatment of diarrhea. dysentery, cholera. ulcer of mouth and wounds. The world-wide increase in the prevalence of antibiotic resistant bacteria requires the rapid development of novel and more potent drugs The unripe matured fruits are successfully used to treat worm infestations of children in Bangladesh. The fruits contain triterpenes, alkanes, Ilavonoids and tannins. The aim of the present study was to investigate antimicrobial activity of methanol and ethanol extract of Diospyros peregrina fruits. Persimmon fruit were examined against some selective gram positive and gram negative bacterial strains. Preliminary antimicrobial activity was evaluated by agar disc diffusion method. Minimum inhibitory concentration was determined by tube dilution (MIC) whilst Minimum Lethal Concentration concentration (MLC). Three organisms were sensitive to Methanol ethanol and w-rater. Persimmon fruit was most effective for Staphylococcus aureus (MIC value 1 125 μg/ml), followed by Vibrio cholerae non 01 (MIC value 1500 μ^g/ml) and Vihriu cholerae 569B (MIC value 2250 μ`g/ml).