Study of oxidative stress on growth and survivability comparison in between laboratory microorganisms and industrial wastewater microorganisms

Abstract

Oxidative stress is one of the most common occurrence on microorganisms whether they are in natural environments, or chemically stressed environments, which typically causes natural cell apoptosis in microbes. ROS or Reactive Oxygen Species is a massive signifier of oxidative stress generation in bacteria apart from their natural aerobic metabolism. However, bacterial organisms have embedded antioxidant properties which can sufficiently tolerate toxic stress levels. Microorganisms contained in chemically stressed environments are highly likely to tolerate hostile environments of stress for survivability, whereas conventional laboratory microorganisms are expected to be less tolerant. There are oxidant reagents available which profoundly generate ROS species responsible for hindering bacterial growth rate at any given circumstances. The purpose of this research is to reinforce ROS generation in both primarily chemically stressed, and non-stressed laboratory bacterial culture samples through external oxidant sources and sketch a thorough comparative analysis between the growth and survivability rates of the mentioned different strains of the similar microbe. Chemically stressed microbes have been accumulated through the collection of semi chemically treated wastewater from the drainage system of manufacturing industries and their correspondent laboratory strains have been simultaneously cultured alongside them. Oxidative stress was induced through external oxidants into all the microorganism through drop spread assay. The results have partially shown as per expectation, however there have been a gigantic amount of unnatural and unexpected scenarios. An assumable level of errors and limitations, and study gaps have been discussed to demonstrate the diversity of results.